0
Scientific Articles   |    
Adipose-Derived Stem-Cell Treatment of Skeletal Muscle Injury
Ramon Peçanha, MsD1; Luiza de Lima e Silva Bagno, MsD2; Marcelo Baldanza Ribeiro, MsD1; Anna Beatriz Robottom Ferreira, PhD3; Milton Ozório Moraes, PhD3; Gisele Zapata-Sudo, PhD4; Taís Hanae Kasai-Brunswick, MsD2; Antônio Carlos Campos-de-Carvalho, PhD2; Regina Coeli dos Santos Goldenberg, PhD2; João Pedro Saar Werneck-de-Castro, PhD1
1 Escola de Educação Física e Desportos-C.C.S., Laboratório de Biologia do Exercício, Departamento de Biociência e Atividade Física, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 540 Ilha do Fundão, Rio de Janeiro, 21941-599, Brazil. E-mail: joaopedrowerneck@yahoo.com.br
2 Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 340, Rio de Janeiro, 21941-902, Brazil
3 Instituto Oswaldo Cruz, FIOCRUZ, Avenida Brasil, 4365, Rio de Janeiro, 21045-900, Brazil
4 Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 340, Rio de Janeiro, 21941-902, Brazil
View Disclosures and Other Information
  • Disclosure statement for author(s): PDF

Investigation performed at Federal University of Rio de Janeiro and Oswaldo Cruz Institute, Rio de Janeiro, Brazil



Disclosure: One or more of the authors received payments or services, either directly or indirectly (i.e., via his or her institution), from a third party in support of an aspect of this work. None of the authors, or their institution(s), have had any financial relationship, in the thirty-six months prior to submission of this work, with any entity in the biomedical arena that could be perceived to influence or have the potential to influence what is written in this work. Also, no author has had any other relationships, or has engaged in any other activities, that could be perceived to influence or have the potential to influence what is written in this work. The complete Disclosures of Potential Conflicts of Interest submitted by authors are always provided with the online version of the article.

Copyright © 2012 by The Journal of Bone and Joint Surgery, Inc.
J Bone Joint Surg Am, 2012 Apr 04;94(7):609-617. doi: 10.2106/JBJS.K.00351
5 Recommendations (Recommend) | 3 Comments | Saved by 3 Users Save Case

Abstract

Background: 

The aim of the present study was to investigate whether adipose-derived stem cells could contribute to skeletal muscle-healing.

Methods: 

Adipose-derived stem cells of male rats were cultured and injected into the soleus muscles of female rats. Two and four weeks after injections, muscles were tested for tetanic force (50 Hz). Histological analysis was performed to evaluate muscle collagen deposition and the number of centronucleated muscle fibers. In order to track donor cells, chimerism was detected with use of real-time polymerase chain reaction targeting the male sex-determining region Y (SRY) gene.

Results: 

Two weeks after cell injection, tetanus strength and the number of centronucleated regenerating myofibers, as well as the number of centronucleated regenerating myofibers, were higher in the treated group than they were in the control group (mean and standard error of the mean, 79.2 ± 5.0% versus 58.3 ± 8.1%, respectively [p < 0.05]; and 145 ± 36 versus 273 ± 18 per 103 myofibers, respectively [p < 0.05]). However, there were no significant differences at four weeks. Treatment did not decrease collagen deposition. Male gene was not detected in female host tissue at two and four weeks after engraftment by polymerase chain reaction analysis.

Conclusions: 

Adipose-derived stem-cell therapy increased muscle repair and force at two weeks, but not four weeks, after injection, suggesting that adipose-derived stem-cell administration may accelerate muscle repair; however, the rapid disappearance of injected cells suggests a paracrine mechanism of action.

Figures in this Article
    Sign In to Your Personal ProfileSign In To Access Full Content
    Not a Subscriber?
    Get online access for 30 days for $35
    New to JBJS?
    Sign up for a full subscription to both the print and online editions
    Register for a FREE limited account to get full access to all CME activities, to comment on public articles, or to sign up for alerts.
    Register for a FREE limited account to get full access to all CME activities
    Have a subscription to the print edition?
    Current subscribers to The Journal of Bone & Joint Surgery in either the print or quarterly DVD formats receive free online access to JBJS.org.
    Forgot your password?
    Enter your username and email address. We'll send you a reminder to the email address on record.

     
    Forgot your username or need assistance? Please contact customer service at subs@jbjs.org. If your access is provided
    by your institution, please contact you librarian or administrator for username and password information. Institutional
    administrators, to reset your institution's master username or password, please contact subs@jbjs.org

    References

    Accreditation Statement
    These activities have been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the joint sponsorship of the American Academy of Orthopaedic Surgeons and The Journal of Bone and Joint Surgery, Inc. The American Academy of Orthopaedic Surgeons is accredited by the ACCME to provide continuing medical education for physicians.
    CME Activities Associated with This Article
    Submit a Comment
    Please read the other comments before you post yours. Contributors must reveal any conflict of interest.
    Comments are moderated and will appear on the site at the discretion of JBJS editorial staff.

    * = Required Field
    (if multiple authors, separate names by comma)
    Example: John Doe





    Related Content
    The Journal of Bone & Joint Surgery
    JBJS Case Connector
    Related Audio and Videos
    PubMed Articles
    Clinical Trials
    Readers of This Also Read...
    JBJS Jobs
    01/22/2014
    PA - Penn State Milton S. Hershey Medical Center
    05/03/2012
    CA - UCLA/OH Department of Orthopaedic Surgery
    03/27/2014
    MA - Boston Medical Center and Boston University School of Medicine
    02/05/2014
    OR - The Center - Orthopedic and Neurosurgical Care and Research