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Scientific Articles   |    
A Mouse Model of Massive Rotator Cuff Tears
Xuhui Liu, MD1; Dominique Laron, MD2; Kyle Natsuhara, BS1; Givenchy Manzano, BS1; Hubert T. Kim, MD, PhD1; Brian T. Feeley, MD1
1 San Francisco Veterans Affairs Medical Center, Building 2, Room 639, 4150 Clement Street, San Francisco, CA 94121. E-mail address for X. Liu: liux@orthosurg.ucsf.edu
2 Department of Orthopaedic Surgery, University of California at San Francisco, 500 Parnassus Avenue, San Francisco, CA 94143
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Investigation performed at the San Francisco Veterans Affairs Medical Center, San Francisco, and the Department of Orthopaedic Surgery, University of California at San Francisco, San Francisco, California



Disclosure: One or more of the authors received payments or services, either directly or indirectly (i.e., via his or her institution), from a third party in support of an aspect of this work. None of the authors, or their institution(s), have had any financial relationship, in the thirty-six months prior to submission of this work, with any entity in the biomedical arena that could be perceived to influence or have the potential to influence what is written in this work. Also, no author has had any other relationships, or has engaged in any other activities, that could be perceived to influence or have the potential to influence what is written in this work. The complete Disclosures of Potential Conflicts of Interest submitted by authors are always provided with the online version of the article.

Copyright © 2012 by The Journal of Bone and Joint Surgery, Inc.
J Bone Joint Surg Am, 2012 Apr 04;94(7):e41 1-10. doi: 10.2106/JBJS.K.00620
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Abstract

Background: 

Rotator cuff tears are the most common tendon injury seen in orthopaedic patients. Muscle atrophy and fatty infiltration in rotator cuff muscles are considered among the key factors responsible for the failure of attempted repair of a massive rotator cuff tear. However, the pathophysiology of rotator cuff muscle atrophy and fatty infiltration remains largely unknown, partly because of the lack of appropriate small animal models. The goal of this study was to develop a mouse model of muscle atrophy and fatty infiltration after a rotator cuff tear. We also sought to study the role of denervation on muscle atrophy and fatty infiltration after a rotator cuff tear.

Methods: 

Adult wild-type FVB/N mice were randomly divided into three groups. Mice in different groups received unilateral complete supraspinatus and infraspinatus tendon transection, suprascapular nerve transection, or both procedures. Sham surgery was performed on the contralateral shoulder to serve as a control. Mice were killed twelve weeks after surgery. Histological analysis and high-resolution magnetic resonance imaging were used to evaluate muscle atrophy and fat infiltration after a rotator cuff tear.

Results: 

Significant and consistent muscle atrophy and fatty infiltration were observed in the rotator cuff muscles after rotator cuff tendon transection. We further found that denervation significantly increases the amount of muscle atrophy and fatty infiltration after a rotator cuff tear.

Conclusions: 

We successfully developed a novel mouse model of a massive rotator cuff tear, which simulates major pathological changes, including muscle atrophy and fatty infiltration after massive rotator cuff tears seen in patients.

Clinical Relevance: 

Successful development of this novel mouse model of rotator cuff tears will provide a powerful tool to study the molecular mechanisms of muscle atrophy and fatty infiltration by introducing transgenic and knockout mice in the future. This model may also serve as a powerful in vivo model in developing new treatments for this common disease.

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    References

    Accreditation Statement
    These activities have been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the joint sponsorship of the American Academy of Orthopaedic Surgeons and The Journal of Bone and Joint Surgery, Inc. The American Academy of Orthopaedic Surgeons is accredited by the ACCME to provide continuing medical education for physicians.
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