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Bone Immunology II. Comparison of Embryonic Mouse Isografts and Homografts
Tawfik Y. Sabet; Erno B. Hidvegi; Robert D. Ray
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Department of Orthopaedic Surgery, Presbyterian-St. Luke's Hospital, and University of Illinois Research and Educational Hospitals, Chicago
1961 by The Journal of Bone and Joint Surgery, Incorporated
J Bone Joint Surg Am, 1961 Oct 01;43(7):1007-1021
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Eighteen-day-old embryonic femora of Strong A mice were transplanted subcutaneously to adult male mice of Strong A strain as isografts and the CBA strain as homografts. Transplants were observed grossly in vivo for three weeks. Host mice were sacrificed at intervals from five to twenty days after transplantation. Vascular, roentgenographic, and histological studies were carried out. A total of 186 specimens was examined of which sixty were isografts, seventy-one homografts, and fifty-five ungrafted controls.

Both isografts and homografts became vascularized between the fourth and fifth day after transplantation. Subsequently, the vascular supply increased in the isografts but decreased in the homografts. The host bed around the isografts remained clear throughout and became hyperemic then returned to normal, whereas the host bed around the homografts was clear, then cloudy, then hyperemic thereafter.

Histologically, endochondral and intramembranous ossification appeared normal in the isografts (although lesser in degree than the controls). The secondary center of ossification for the femoral condyles appeared at the normal time, but later endochondral ossification decreased until it became inactive. In the homografts the marrow was replaced by loose undifferentiated cells, the epiphyseal disc became disorganized, endochondral ossification was disrupted with cartilaginous rests persisting in the diaphysis, and vascularization of the secondary center of ossification of the condyles was delayed. Infiltration with small round cells was noticeable both inside the grafts, as well as in the host beds surrounding them.

From these results it is concluded that embryonic mouse femur from one inbred strain possesses transplantation antigens that can invoke a tissue immune response in adult mice of a different inbred strain.

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    Accreditation Statement
    These activities have been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the joint sponsorship of the American Academy of Orthopaedic Surgeons and The Journal of Bone and Joint Surgery, Inc. The American Academy of Orthopaedic Surgeons is accredited by the ACCME to provide continuing medical education for physicians.
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