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Scientific Articles   |    
Intercellular Adhesion Molecule-1 (ICAM-1, CD54) Is Increased in Adhesive Capsulitis
Yang-Soo Kim, MD1; Jung-Man Kim, MD1; Yun-Gyoung Lee, MS1; Oak-Kee Hong, PhD1; Hyuk-Sang Kwon, MD1; Jong-Hoon Ji, MD2
1 Department of Orthopedic Surgery (Y.-S.K., J.-M.K., and Y.-G.L.) and Department of Endocrinology (O.-K.H. and H.-S.K.), Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul 137-701, South Korea. E-mail address for Y.-S. Kim: kysoos@catholic.ac.kr
2 Department of Orthopedic Surgery, Daejoen St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, 520-2 Daeheung-dong, Joong-gu, Daejoen 301-723, South Korea. E-mail address: junwoo0220@yahoo.co.kr
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Investigation performed at the Department of Orthopedic Surgery and Department of Endocrinology, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, and the Department of Orthopedic Surgery, Daejoen St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Daejoen, South Korea



Disclosure: One or more of the authors received payments or services, either directly or indirectly (i.e., via his or her institution), from a third party in support of an aspect of this work. None of the authors, or their institution(s), have had any financial relationship, in the thirty-six months prior to submission of this work, with any entity in the biomedical arena that could be perceived to influence or have the potential to influence what is written in this work. Also, no author has had any other relationships, or has engaged in any other activities, that could be perceived to influence or have the potential to influence what is written in this work. The complete Disclosures of Potential Conflicts of Interest submitted by authors are always provided with the online version of the article.

Copyright © 2013 by The Journal of Bone and Joint Surgery, Inc.
J Bone Joint Surg Am, 2013 Feb 20;95(4):e18 1-8. doi: 10.2106/JBJS.K.00525
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Abstract

Background: 

The purpose of this study was to investigate the presence of intercellular adhesion molecule-1 (ICAM-1) in shoulders with adhesive capsulitis (“frozen shoulder”).

Methods: 

Glenohumeral capsular tissue was obtained from twenty-six patients (seventeen with adhesive capsulitis and nine controls), and ICAM-1 was evaluated with use of oligonucleotide arrays, real-time reverse transcription-polymerase chain reaction (RT-PCR), and immunohistochemistry. ICAM-1 was also evaluated in synovial fluid with use of western blotting (six patients with adhesive capsulitis and two controls) and in peripheral blood with use of an enzyme-linked immunosorbent assay (ELISA) (thirty-two patients with adhesive capsulitis, twenty with diabetes mellitus, and fourteen controls). The effect of ICAM-1 treatment on gene expression of cytokines related to inflammation and fibrosis was evaluated in cultured normal human synovial cells.

Results: 

The level of ICAM-1 was significantly greater in capsular tissue from the glenohumeral joint of patients with adhesive capsulitis compared with controls as measured by oligonucleotide array analysis (0.12 ± 0.01 compared with 0.09 ± 0.00 arbitrary units) (p = 0.001), real-time RT-PCR (1.70 ± 0.19 compared with 0.67 ± 0.24 arbitrary units) (p < 0.05), and immunohistochemical staining. ICAM-1 was also significantly increased in the synovial fluid of patients with adhesive capsulitis (1.70 ± 0.18 arbitrary units) compared with normal controls (0.48 ± 0.17) (p < 0.05) and in serum of patients with adhesive capsulitis (633.22 ± 59.14 ng/mL) and patients with diabetes mellitus (671.25 ± 27.08 ng/mL) compared with controls (359.86 ± 44.29 ng/mL) (p < 0.05). Gene expression of cytokines related to inflammation and fibrosis in synoviocytes cultured in vitro was greater after three days of treatment with ICAM-1 and with ICAM-1 with glucose compared with untreated cells.

Conclusions: 

ICAM-1 was increased in patients with adhesive capsulitis, similar to the increase that has been reported in patients with diabetes mellitus.

Clinical Relevance: 

ICAM-1 may be a therapeutic target for treating adhesive capsulitis.

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    Accreditation Statement
    These activities have been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the joint sponsorship of the American Academy of Orthopaedic Surgeons and The Journal of Bone and Joint Surgery, Inc. The American Academy of Orthopaedic Surgeons is accredited by the ACCME to provide continuing medical education for physicians.
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