Dupuytren contracture is a disease of the palmar fascia characterized by
nodular fibroblastic proliferation; its etiology and pathogenesis are
poorly understood. Growth factors are polypeptides that regulate cell
growth and differentiation and extracellular matrix production.
Platelet-derived growth factor is known to cause fibroblastic
proliferation, and it may be involved in the pathogenesis of Dupuytren
contracture. The purpose of this study was to determine if the gene for the
B chain of platelet-derived growth factor is expressed in Dupuytren
contracture. Tissue from patients who had Dupuytren disease was examined
immunohistochemically with the 5B5 antibody, which is a marker for
fibroblasts. Polymerase chain reaction, gel electrophoresis, Southern
blotting, and in situ hybridization were also used to study gene expression
in the tissue as well as in normal fascia, A172 cells, and MRC5 cells.
Total cellular RNA was extracted from tissue and cells. Polymerase chain
reaction was done with oligonucleotide primers complementary to a portion
of the platelet-derived growth-factor-B and platelet-derived
growth-factor-receptor genes. The platelet-derived growth-factor-B gene was
expressed in all six specimens from the patients who had Dupuytren
contracture as well as in the A172 cells, but not in the normal fascia lata
or the MRC5 cells. These results were confirmed with Southern blotting of
the products of the reaction with a platelet-derived growth-factor-B probe.
The gene for the platelet-derived growth-factor receptor was expressed by
all tissues and cells studied.(ABSTRACT TRUNCATED AT 250 WORDS)