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Scientific Articles   |    
Effects of Autologous Platelet-Rich Plasma on Cell Viability and Collagen Synthesis in Injured Human Anterior Cruciate Ligament
Louay Fallouh, MD, PhD1; Koichi Nakagawa, MD, PhD2; Takahisa Sasho, MD, PhD1; Momoko Arai, ME1; Sota Kitahara, MD, PhD1; Yuichi Wada, MD, PhD3; Hideshige Moriya, MD, PhD1; Kazuhisa Takahashi, MD, PhD1
1 Department of Orthopaedic Surgery, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan
2 Department of Orthopaedic Surgery, Sakura Medical Center, Toho University, 564-1 Shimoshizu, Sakura, Chiba 285-8741, Japan. E-mail address: konakag@aol.com
3 Department of Orthopaedic Surgery, Chiba Medical Center, Teikyo University, 3426-3 Anegasaki, Ichihara 299-0111, Japan
View Disclosures and Other Information
Disclosure: In support of their research for or preparation of this work, one or more of the authors received, in any one year, outside funding or grants of less than $10,000 from DePuy Japan and the Japan Society for the Promotion of Science (Grant-in-Aid for Scientific Research [B] 17390408). Neither they nor a member of their immediate families received payments or other benefits or a commitment or agreement to provide such benefits from a commercial entity.

Investigation performed at Chiba University, Chiba, Japan

Copyright © 2010 by The Journal of Bone and Joint Surgery, Inc.
J Bone Joint Surg Am, 2010 Dec 15;92(18):2909-2916. doi: 10.2106/JBJS.I.01158
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Abstract

Background: 

Platelet-rich plasma is a fraction of plasma in which platelets are concentrated. It is reported to represent a source of multiple growth factors that promote tissue repair. In anticipation of the eventual testing of platelet-rich plasma in anterior cruciate ligament (ACL)-deficient patients, we examined the effect of autologous platelet-rich plasma on human ACL cell function in vitro.

Methods: 

Fresh blood and ACL remnants were obtained from four patients who underwent ACL reconstruction surgery. Platelet-poor plasma and platelet-rich plasma were prepared from the blood samples. The concentrations of various growth factors in each preparation were tested with use of enzyme-linked immunosorbent assays. Isolated ACL cells were cultured in the presence of 5% fetal bovine serum, 5% platelet-poor clot releasate, 5% platelet-rich clot releasate, or 10% platelet-rich clot releasate. Platelet-rich plasma and platelet-poor plasma releasates were applied to the ACL cells from the same patient autologously. Cell viability and collagen synthesis in each group were analyzed, and semiquantitative gene-expression assays for type-I and III collagen were also performed.

Results: 

The concentrations of the main growth factors (transforming growth factor-beta, platelet-derived growth factor, epidermal growth factor, and vascular endothelial growth factor) were much higher in platelet-rich clot releasate than in platelet-poor clot releasate. In vitro treatment of ACL cells with platelet-rich clot releasate resulted in a significant increase in cell number compared with platelet-poor clot releasate. Total collagen production by the platelet-rich clot releasate-treated cells was significantly higher than that of the platelet-poor clot releasate-treated cells only because of enhanced cell proliferation. There was no significant effect of platelet-rich clot releasate treatment on gene expression for type-I collagen, but expression of type-III collagen was significantly enhanced by the treatment with platelet-rich clot releasate.

Conclusions: 

These results suggest that autologous platelet-rich plasma can enhance ACL cell viability and function in vitro.

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    Accreditation Statement
    These activities have been planned and implemented in accordance with the Essential Areas and policies of the Accreditation Council for Continuing Medical Education (ACCME) through the joint sponsorship of the American Academy of Orthopaedic Surgeons and The Journal of Bone and Joint Surgery, Inc. The American Academy of Orthopaedic Surgeons is accredited by the ACCME to provide continuing medical education for physicians.
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