RT Journal
A1 Peçanha, Ramon
A1 Bagno, Luiza de Lima e Silva
A1 Ribeiro, Marcelo Baldanza
A1 Robottom Ferreira, Anna Beatriz
A1 Moraes, Milton Ozório
A1 Zapata-Sudo, Gisele
A1 Kasai-Brunswick, Taís Hanae
A1 Campos-de-Carvalho, Antônio Carlos
A1 Goldenberg, Regina Coeli dos Santos
A1 Saar Werneck-de-Castro, João Pedro
T1 Adipose-Derived Stem-Cell Treatment of Skeletal Muscle Injury
JF The Journal of Bone & Joint Surgery
JO The Journal of Bone & Joint Surgery
YR 2012
FD April 4
VO 94
IS 7
SP 609
OP 617
DO 10.2106/JBJS.K.00351
UL http://dx.doi.org/10.2106/JBJS.K.00351
AB Background: 
  The aim of the present study was to investigate whether adipose-derived stem cells could contribute to skeletal muscle-healing.Methods: 
  Adipose-derived stem cells of male rats were cultured and injected into the soleus muscles of female rats. Two and four weeks after injections, muscles were tested for tetanic force (50 Hz). Histological analysis was performed to evaluate muscle collagen deposition and the number of centronucleated muscle fibers. In order to track donor cells, chimerism was detected with use of real-time polymerase chain reaction targeting the male sex-determining region Y (SRY) gene.Results: 
  Two weeks after cell injection, tetanus strength and the number of centronucleated regenerating myofibers, as well as the number of centronucleated regenerating myofibers, were higher in the treated group than they were in the control group (mean and standard error of the mean, 79.2 ± 5.0% versus 58.3 ± 8.1%, respectively [p < 0.05]; and 145 ± 36 versus 273 ± 18 per 103 myofibers, respectively [p < 0.05]). However, there were no significant differences at four weeks. Treatment did not decrease collagen deposition. Male gene was not detected in female host tissue at two and four weeks after engraftment by polymerase chain reaction analysis.Conclusions: 
  Adipose-derived stem-cell therapy increased muscle repair and force at two weeks, but not four weeks, after injection, suggesting that adipose-derived stem-cell administration may accelerate muscle repair; however, the rapid disappearance of injected cells suggests a paracrine mechanism of action.